Jannasch
nov., a novel haloalkaliphile isolated from a soda lake in Xinjiang, China. Under anaerobic conditions, the rate of sulfide oxidation was very low. All of the isolates were obligately heterotrophic. E.
Solid dithionite was used for the complete reduction of cytochromes, and bubbling with air for their oxidation. P.A.
Robertson
Li
(, Bennasar
Direct DNA-DNA hybridization between all isolates was therefore possible. Can you explain the synthesis and reaction of a Grignard Reagent. By continuing you agree to the use of cookies. The synthesis of ATP during the oxidation of thiosulfate to tetrathionate in washed suspensions of cells from several strains has been reported [34]. Sequences were aligned according to secondary structure in the sequence editor SeqPup [27].
Cytochrome b was present at relatively low concentrations, compared to the level of cytochrome c. CO difference spectra of dithionite-reduced extracts had the maxima and minima in the γ region (415 and 432 nm respectively), and minima in the α region (556–558 nm) typical of cytochrome oxidase type o. In this study, tetrathionate was generated in a ROS‐dependent process during inflammation, likely by oxidation of thiosulfate present in the gut. P.W.J.
/Linearized 1.0 However, DNA-DNA hybridization on R and S clones from each of three of the strains (TG 3, BG 2 and ChG 5-2) confirmed that in each case, the clones were the same organism (hybridization level 99–100%). Less nitrite accumulated in nitrate-reducing cultures containing thiosulfate and acetate, presumably because of the changed electron donor:acceptor ratio. Most of the hybridization and G+C mol% measurements were done using the thermal denaturation technique [21]. It is to be expected, as techniques are refined, that other strains will be classified, upon closer examination, as P. stutzeri. Institute of Microbiology, Russian Academy of Sciences, Prospect 60-let Octyabrya 7/2, 117811 Moscow, Russia.
This is a preview of subscription content, log in to check access. McCaughey
M.
Washed cells of ‘F. Unless otherwise mentioned, 10–20 mM acetate, 10 mM thiosulfate and 10–20 mM of nitrite or nitrate were used. (, Vedenina
Copyright © 1985 Published by Elsevier B.V. https://doi.org/10.1016/S0166-9834(00)82668-8. Phylogenetic tree based on 16S rRNA sequence positions 34–1461 for P. stutzeri and related strains, including the new thiosulfate-oxidizing isolates described in this study. In addition, the 3′ ends of the 16S rDNA amplificates were re-sequenced manually, as described previously [24], to check the often minute sequence differences between the strains. These experiments also demonstrated that the culture could not gain sufficient energy from thiosulfate oxidation to increase the growth yield observed with acetate alone. This work received financial support from the Max-Planck-Gesellschaft, Munich to A.T. Tuttle
G.J. Strains ChG 6-1 and ChG 5-3 differed from the others by their ability to utilize d-galacturonic and d-glucuronic acids.
PHYLIP (Phylogeny Inference Package 3.5c), Department of Genetics, University of Washington, A place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology, Taxonomic note: a pragmatic approach to the nomenclature of phenotypically similar genomic groups, 16S rRNA gene sequence analysis relative to genomovars of, Strain JM300 represents a new genomovar within, ATP synthesis by heterotrophic bacteria during thiosulfate oxidation to tetrathionate, Effects of sulfide and acetylene on nitrous oxide reduction by soil and, The bacterial populations and processes of sulfide oxidation in the Black Sea, A denitrifying bacterium from the deep sea at 11000 m depth, Microbial flora in the deepest sea mud of the Mariana Trench, © 1999 Federation of European Microbiological Societies. Overbeek
(, Maidak
Difference spectra of cell-free extracts prepared from strains from group 1 showed the presence of cytochrome cd1. Dendrogram of the DNA-DNA homology among strains capable of thiosulfate-dependent denitrification. Most of the strains were isolated from the interface micro-aerobic layer of the Black Sea where they represent the dominant sulfur-oxidizing community [2,4]. J Biol Chem 193:265–275, PubMed Laculat
The morphology of the cells within the R- and S-type colonies was sufficiently different to suggest two different species. 0000000015 00000 n The Ribosomal Database Project [25] and GenBank [26] were searched for sequences of closely related strains. Subscription will auto renew annually. Oxidation of thiosulfate in batch cultures of tetrathionate-forming heterotrophic bacteria grown with different electron acceptors. A Biosequence Editor and Analysis Application.
Robertson
However, in the presence of chalcopyrite which contains ferric iron, thiosulfate is rapidly and quantitatively oxidized to tetrathionate which then undergoes slow oxidation to sulfate. The chief was seen coughing and not wearing a mask.
Some strains converted thiosulfate to tetrathionate equally well with nitrite, nitrate and N2O as electron acceptors. Chemolithoautotrophic oxidation of thiosulfate, tetrathionate and thiocyanate by a novel rhizobacterium belonging to the genus Paracoccus. oxidation of thiosulfate by iodine or other oxidants typically gives tetrathionate.23 Assuming that r; = r t, this oxidation mechanism gives a rate equation of the form: By substituting the equilibrium of sulfite and bisulfite and assuming that S(IV) is mostly bisulfite, valid for pH less