Mutant EF-3, after exposure to a nonpermissive temperature, failed to stimulate binding of the ternary complex of EF-1 X GTP X aminoacyl-tRNA to ribosome. Additionally, the isolated radioactive fragments were further cleaved at Trp residues and sequenced. Journal of Biological Chemistry | Citations: 396,051 | Complete content of the Journal of Biological Chemistry as of April 1995. Several analogs of orcinol also serve as substrates for hydroxylation, namely resorcinol, 4-methylresorcinol, and 4-bromoresorcinol. subunit-binding site disulfide, Regulatory volume decrease in the presence of HCO3 - by single osteosarcoma cells UMR-106-01, Metabolism of resorcinylic compounds by bacteria. Silver stain analysis of the sodium dodecyl sulfate electrophoretograms indicated that the affinity-purified protein was better than 90% pure. This glycoprotein was purified, and amino-terminal sequence analysis identified it as bone sialoprotein (BSP). were obtained in the sequencing cycles corresponding to Asp, Asp, and Glu. The bound and free oligonucleotides were then subjected to nuclease protection assays using DNase I and a complex of 1,10-phenanthroline-copper. The technique for the simultaneous recording of cell volume changes and pHi in single cells was used to study the role of HCO3- in regulatory volume decrease (RVD) by the osteosarcoma cells UMR-106-01. In platelets, the primary site of PGHS-1 synthesis is in precursor megakaryocytic cells. U. S. A 90, 6285-6289). The mutation of Asp to Asn decreased the affinity of the receptor for ACh 100-fold, whereas the mutation of either Asp, Glu, or 8 other acidic residues in the same region of decreased the affinity by 3-fold or less (Czajkowski, C., Kaufmann, C., and Karlin, A. R 59 022 had no effect on the breakdown of phosphoinositides. Residual contaminating proteins and nucleic acids can be removed by gel filtration, but an even simpler final purification is possible, because under appropriate conditions the 0.3 protein is soluble in high concentrations of ethanol. Pyruvate, oxalacetate, and oxalate are competitive inhibitors of acetylpyruvate hydrolysis by the enzyme with K-i values of 6.0, 4.5, and 0.45 mM, respectively. regulation. Journal of Biological Chemistry is cited by a total of 26803 articles during the last 3 years (Preceding 2018). The hydrophobic probe, 1,5-AZNS, also interacted with both subunits of alpha-crystallin. The minimal structural requirements for effectors appear to be a 1,3-dihydroxy or 1-alkyl-3-hydorxybenzene, but only the former are substrates for hydroxylation. The enzyme has been purified approximately 40-fold from extracts of Ps. The mutant 0.3 proteins that contain 87 or more amino acids appear to be reasonably stable in vivo, but those that contain 78 or fewer are apparently too unstable to have been observed by gel electrophoresis. Identification of acidic residues in the ?? A reporter gene system was established to assess the role of Galpha16 on erythroid differentiation of MB-02 erythroleukemia cells. ACM Localization of 1,5-AZNS binding site in alphaB-crystallin lead to the identification of HFSPEEK sequence as the interacting site in this subunit of alpha-crystallin. Two different methods were used to determine the number of Bohr protons released upon oxygenation of human hemoglobin (Hb A) and Hb A lacking beta 146 His (des-His Hb A) at the pH ranging from pH 5.0 to 9.0 in the presence of 0.1 M Cl- at 25 degrees C. One is the direct differential titration method, the other is based on the measurement of oxygen affinity as a function of pH. The enzyme therefore behaves either as a hydroxylase or an oxidase. ethylenediamine or 6-aminohexanoic acid. Oligosaccharide analyses indicated that for every N-linked oligosaccharide on the BSP, there are also approximately 2 hexa-, approximately 5 tetra-, and approximately 2 trisaccharides O-linked to serine and threonine residues. This large number of oligosaccharides is in agreement with the known carbohydrate content (approximately 50%) of the BSP. In contrast, the peptide bond after Val(279) is a minor cleavage site, influenced by both distal and proximal amino acid residues. However, the rules governing the specificity of cleavage sites are still largely unknown. Chem. (1991) J. Biol. The apparent K-m values for the effectors are in good agreement with the D-D values obtained for orcinol, resorcinol, and m-cresol. undergoing clinical trials for cancer treatment, abrogates G2 checkpoint function and sensitizes p53-defective cancer cells to DNA-damaging agents. Heterotrimeric G proteins may assume modulatory roles in cellular proliferation and differentiation. The visible absorption spectrum of the enzyme shows maxima at 373 and 454 nm and a shoulder at 480 nm. It is used for the recognition of journals, newspapers, periodicals, and magazines in all kind of forms, be it print-media or electronic. Evidence for maximum values of 1.0, 0.5, and 1.0 at sites 1, 2, and 3, Purification and characterization of a dialyzable 0.6 S 2 -globulin from normal human plasmgulation of the biosynthesis of enzymes utilized in germination, Crystal Structure of Triosephosphate Isomerase Complexed with 2-Phosphoglycolate at 0.83-A Resolution, Structure of the nicotinic receptor acetylcholine-binding site. A checkpoint operating in the G2 phase of the cell cycle prevents entry into mitosis in the presence of DNA damage. The ASBMB is pleased to announce that the society’s three journals — Journal of Biological Chemistry, Molecular & Cellular Proteomics and Journal of Lipid Research — will be fully open access beginning in January 2021. However, the production induced by phorbol 12-myristate 13-acetate (PMA), a direct activator for protein kinase C, was not potentiated by R 59 022. These results also provide further evidence for the close relationship between 46-kDa protein phosphorylation by protein kinase C and stimulation of O2- production in PMNL. The activated matrix (an imidazolyl carbamate) is relatively stable to hydrolysis but smoothly reacts with N-nucleophiles such as those present in either affinity chromatography ligands or leashes, e.g.